Apoptosis and expression of argyrophilic nucleolus organizer regions in epithelial neoplasms of the larynx

作者：Ribeiro Christiana Vargas^*; Vasconcelos Anilton Cesar; Andrade Filho Jose de Souza

来源：Brazilian Journal of Otorhinolaryngology, 2015, 81(2): 158-166.

DOI：10.1016/j.bjorl.2014.12.003

• Apoptosis
• Larynx
• Squamous cell carcinoma
• Nucleolus organizer region
• Papilloma

Introduction: Occurrence of apoptosis and expression of proliferative markers are powerful tools to establish a prognosis in the follow-up of cancer.
Objective: To evaluate the growth fraction in papillomas and laryngeal squamous cell carcinomas with three degrees of differentiation through apoptosis and the expression of nucleolus organizer regions.
Methods: Retrospective study from which paraffin material was submitted to microtomy and hematoxylin-eosin and silver staining. Stained slides were used to quantify the apoptotic index and the number of nucleolus organizer regions by morphometry.
Results: Apoptosis was significantly more frequent in well differentiated carcinomas and in papillomas, and a higher growth fraction of expressed nucleolus organizer regions and cells that expressed a greater than average number of nucleolus organizer regions were more frequently noted in undifferentiated carcinomas.
Conclusions: Thus, it was possible to verify that a high apoptotic index was associated with a lower chance of tumor differentiation in carcinomas, while a greater number of total nucleolus organizer regions, cells expressing nucleolus organizer regions above average and a higher growth fraction were associated with greater likelihood of abnormal cell proliferation and increased tumor differentiation.

• 出版日期2015-4