Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly

作者：White Simon J^*; Johnson Steven; Szymonik Michal; Wardingley Richard A; Pye Douglas; Davies A Giles; Waelti Christoph; Stockley Peter G

来源：Nanotechnology, 2012, 23(49): 495304.

DOI：10.1088/0957-4484/23/49/495304

Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using self-assembly of the peptide coiled-coil heterodimer ACID:BASE to immobilize M13 bacteriophage particles to specific locations on a patterned gold surface. Surface plasmon resonance demonstrated that free ACID peptides will assemble onto a surface derivatized with BASE. We then displayed the ACID peptide on the pIX coat protein of M13 and showed that these phage particles permit formation of the coiled-coil resulting in specific surface attachment. The ACID: immobilized BASE affinities appear to be similar for free peptide and phage-displayed ACID. Finally, we fabricated two gold electrodes, separated by a 200 nm gap, coated one of them with BASE and showed that this allows localization of the M13:ACID onto the functionalized electrode.

• 出版日期2012-12-14