Fas/FasL-mediated apoptosis in the arcuate nucleus and medial preoptic area of male ArKO mice is ameliorated by selective estrogen receptor alpha and estrogen receptor beta agonist treatment, respectively

作者：Hill Rachel A; Chow Jenny; Fritzemeier Karlheinrich; Simpson Evan R; Boon Wah Chin^*

来源：Molecular and Cellular Neuroscience, 2007, 36(2): 146-157.

DOI：10.1016/j.mcn.2007.05.012

The aromatase (ArKO) knockout mouse is estrogen deficient. Our previous analysis revealed apoptosis of dopaminergic neurons in the arcuate nucleus (Are) and medial preoptic area (MPO) of 1-year-old male ArKO mice. We sought to determine which estrogen receptor (ER) is involved in the anti-apoptotic action of estrogen. Male ArKO (9.5-month-old) mice were treated with 16 alpha-LE2 (ER alpha-specific agonist) or 8 beta-VE2 (ER beta-specific agonist). Daily injections (6 weeks) with 160(LE, prevented dopaminergic cell death in the Arc of male ArKO mice, with no significant effect of 8 beta-VE2 treatment. In contrast, 8 beta-VE2 prevented dopaminergic cell death in the MPO, while 16ot-LE had no significant effect. Concomitant decreases in Fas and FasL protein levels were found upon 16 alpha-LE2 and 8 beta-VE2 treatment in the Are and MPO, respectively. Our results indicate that anti-apoptotic effects of estrogen are ER mediated, and the specific ER subtype involved in regulating apoptosis depends on the particular brain nucleus in question.

• 出版日期2007-10
• 单位河北医科大学