PLC epsilon knockdown inhibits prostate cancer cell proliferation via suppression of Notch signalling and nuclear translocation of the androgen receptor

作者：Wang Yin; Wu Xiaohou; Ou Liping; Yang Xue; Wang Xiaorong; Tang Min; Chen E; Luo Chunli^*

来源：Cancer Letters, 2015, 362(1): 61-69.

DOI：10.1016/j.canlet.2015.03.018

• PLC epsilon
• Notch1
• Androgen receptor
• Prostate cancer
• Cell proliferation

Phospholipase C epsilon (PLC epsilon), a key regulator of diverse cellular functions, has been implicated in various malignancies. Indeed, PLC epsilon functions include cell proliferation, apoptosis and malignant transformation. Here, we show that PLCe expression is elevated in prostate cancer (PCa) tissues compared to benign prostate tissues. Furthermore, PLC epsilon depletion using an adenovirally delivered shRNA significantly decreased cell growth and colony formation, arresting the PC3 and LNCaP cell lines in the S phase of the cell cycle. We also observed that PLC epsilon was significantly correlated with Notch1 and androgen receptor (AR). Additionally, we demonstrate that the activation of both the Notch and AR signalling pathways is involved in PLC epsilon-mediated oncogenic effects in PCa. Our findings suggest that PLC epsilon is a putative oncogene and prognostic marker, potentially representing a novel therapeutic target for PCa.

• 出版日期2015-6-28
• 单位重庆医科大学