Anterior cruciate ligament-derived cells have high chondrogenic potential

作者：Furumatsu Takayuki^*; Hachioji Motomi; Saiga Kenta; Takata Naoki; Yokoyama Yusuke; Ozaki Toshifumi

来源：Biochemical and Biophysical Research Communications, 2010, 391(1): 1142-1147.

DOI：10.1016/j.bbrc.2009.12.044

• Anterior cruciate ligament
• Medial collateral ligament
• Chondrogenesis
• Sox9
• Scleraxis
• Differentiation

Anterior cruciate ligament (ACL)-derived cells have a character different from medial collateral ligament (MCL)-derived cells. However, the critical difference between ACL and MCL is still unclear in their healing Potential and cellular response. The objective of this study was to investigate the mesenchymal differentiation property of each ligament-derived cell. Both ligament-derived cells differentiated into adipogenic, osteogenic, and chondrogenic lineages. In chondrogenesis, ACL-derived cells had the higher chondrogenic property than MCL-derived cells. The chondrogenic marker genes, Sox9 and alpha 1(II) collagen (Col2a1), were induced faster in ACL-derived pellets than in MCL-derived pellets. Sox9 expression preceded the increase of Col2a1 in both pellet-cultured cells. However, the expression level of Sox9 and a ligament/tendon transcription factor Scleraxis did not parallel the increase of Col2a1 expression along with chondrogenic induction. The present Study demonstrates that the balance between Sox9 and Scleraxis have an important role in the chondrogenic differentiation of ligament-derived cells.

• 出版日期2010-1-1