Downregulating PRL-3 inhibit migration and invasion of lung cancer cell via RhoA and mDia1

作者：Ming Jian; Liu Nan; Jiang Guocheng; Zhang Qingfu; Qiu Xueshan^*; Wang Enhua

来源：TUMORI JOURNAL, 2012, 98(3): 370-376.

DOI：10.1177/030089161209800315

• PRL-3
• RhoA
• mDia1
• migration
• invasion

Aims and background. The overexpression of phosphatase of regenerating liver-3 (PRL-3) affects the migration and invasion of epithelial cells. Little is known about the substrates or pathways that PRL-3 interacts with.
Methods. We studied the action and the mechanism of PRL-3 in migration and invasion of lung cancer. We detected the expression of PRL-3 in lung cancer cell lines and normal human bronchial epithelial cell.
Results. We found that PRL-3 expression was high in lung cancer cells. Knockdown of PRL-3 by siRNA inhibited cell migration and invasion and reorganizated the cytoskeleton. Furthermore, blocking PRL-3 decreased RhoA activity and mDia1 expression. Blocking RhoA or mDia1 showed the similar changes of cytoskeleton and suppression of migration and invasion as inhibiting PRL-3 expression. Blocking RhoA inhibited the expression of mDia1.
Conclusions. These data indicate that downregulating PRL-3 inhibit cell migration and invasion by inactivating RhoA to downregulate mDia1 to reorganizate cytoskeleton of lung cancer cells.

• 出版日期2012-6
• 单位中国医科大学; 中国人民解放军第二O二医院