Detection and characterization of a new betasatellite: variation in disease symptoms of tomato leaf curl Pakistan virus-India due to associated betasatellite

作者：Kumar Jitendra^*; Singh Sudhir P; Kumar Abhinav; Khan Jawaid A; Tuli Rakesh

来源：Archives of Virology, 2013, 158(1): 257-261.

DOI：10.1007/s00705-012-1486-5

• Geminivirus
• Begomovirus
• Betasatellite
• Phylogenetic analysis
• Recombination

A begomovirus and its associated betasatellites were amplified and sequenced from tobacco plants affected with leaf curl disease. The begomovirus was identified as a new strain of tomato leaf curl Pakistan virus (ToLCPKV), which is referred to here as ToLCPKV-India. A previously known betasatellite [tomato leaf curl Patna betasatellite (ToLCPaB)] and a new betasatellite were also found in leaf-curl-affected samples. The use of infectious clones of ToLCPKV-IN plus ToLCPaB for agroinoculation led to typical leaf curl, while ToLCPKV-IN together with the new betasatellite resulted in curling and chlorosis of leaves. Based on these disease symptoms, we propose to name the new betasatellite tobacco leaf chlorosis betasatellite (TbLChB).

• 出版日期2013-1