A novel rat model combining surgical vein grafting with type 2 diabetes

作者：Huo, Tianming; Zhang, Jueyu; Lan, Zhicun; Feng, Zhiqiang; Huang, Qiangxin; Liang, Ludong; Zhang, Bo; Chen, Gang^*; Li, Shikang^*

来源：International Journal of Clinical and Experimental Medicine, 2016, 9(8): 15306-15315.

• Coronary artery disease
• type 2 diabetes
• rat model
• neointima
• surgery

Background: Diabetes is a risk factor for vein graft failure after bypass surgery with coronary artery disease (CAD). We first developed a vein grafting model in rats with a type 2 diabetes mellitus-like (T2DM) condition to provide animal models for relevant clinical research. Methods: Sixty male Sprague-Dawley rats were fed high-fat (T2DM group) or normal food (CON group) for one month and then injected (T2DM group) with streptozotocin (35 mg/kg) to induce T2DM. Insulin or glucose tolerance tests were performed to verify the T2DM models. All rats subsequently underwent surgical vein grafting, and the morphology of their grafts at 0, 14, and 28 days was analyzed under microscope. Results: The T2DM group exhibited significantly increased glucose, insulin, free fatty acids, and cholesterol levels (all, P < 0.05), and also exhibited significant hyperglycemic and hyperinsulinemic responses to an intraperitoneal glucose tolerance test compared to that in the CON group. Furthermore, the insulin sensitivity of the T2DM group was significantly reduced as the CON group gained little change according to an insulin tolerance test (P < 0.05). Additionally, the number of cell nuclei of the intima and media on the 14th and 28th day in the T2DM group approximately doubled as compared to that in the CON group (both, P < 0.05). Conclusion: We successfully developed a model of surgical vein grafting in type 2 diabetic rats. This novel rat model may facilitate in vivo mechanistic studies of the increased neointimal formation in type 2 diabetes mellitus-related vein graft failure.

• 出版日期2016
• 单位广西医科大学