Determination of Antioxidant and Anti-Melanogenesis Activities of Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk) Becc] Fruit Extract

作者：Arung Enos Tangke^*; Suwinarti Wiwin; Hendra Medi; Supomo; Kusuma Irawan Wijaya; Puteri Dwi Caesarina Nanda; Eroglu Huseyin Avni; Kim Yong Ung; Shimizu Kuniyoshi; Ishikawa Hiroya

来源：Tropical Journal of Pharmaceutical Research, 2015, 14(1): 41-46.

DOI：10.4314/tjpr.v14i1.7

• Durio kutejensis
• Antioxidant
• Anti-melanogenesis
• B16 Melanoma cell Hyperpigmentation
• Skin-lightening agent

Purpose: To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract.
Methods: The fruit flesh of Durio kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity).
Results: The extract (200 mu g/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47% without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 mu g/mL) also exhibited some level of antioxidant activity including ORAC (0.04 +/- 0.00 mu mol TE/mg at 950 mu g/mL), ABTS (1.0 +/- 0.2% at 100.8 mu g/mL), SOD (IC50, 76.00 +/- 14.6 mu g/mL, and DPPH (21.5 +/- 0.7% at 97.39 +/- g/mL extract concentration).
Conclusion: The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.

• 出版日期2015-1