Anti-proliferative and apoptosis-inducing activities of juglone in LS-174T cells

作者：Li Qiang; Zhao Xue Ling; Sun Juan; Jiang Shou Gang; Gong Xian Feng^*

来源：Bangladesh Journal of Pharmacology, 2013, 8(1): 65-72.

DOI：10.3329/bjp.v8i1.13174

• Apoptosis
• Juglone
• LS-174T cell
• NAC

Anti-proliferative and apoptosis-inducing effects of juglone in LS-174T cells were investigated. In this study, we showed that juglone inhibited the proliferation of LS-174T cells in a time and dose-dependent manner, treatment of juglone resulted in the activation of caspase-9 and caspase-3, decrease of Bcl-2. N-acetylcysteine significantly attenuate LS-174T cell death induced by juglone (p<0.001). In addition, NAC could reverse caspase-3 and caspase-9 activation, increase expression of Bcl-2 protein. Taken together, these findings indicated that juglone-mediated oxidative injury may act as upstream change, trigger ROS release, Bcl-2 modulation, caspase activation, and consequently leading cell apoptosis in LS-174T cells. In conclusion, these findings suggest that juglone may be an effective way for treating human cancers.

• 出版日期2013
• 单位东北林业大学; 黑龙江大学