Activity and intracellular location of estrogen receptors alpha and beta in human bronchial epithelial cells

作者：Ivanova Margarita M; Mazhawidza Williard; Dougherty Susan M; Minna John D; Klinge Carolyn M^*

来源：Molecular and Cellular Endocrinology, 2009, 305(1-2): 12-21.

DOI：10.1016/j.mce.2009.01.021

• Estrogen receptor
• Lung adenocarcinoma (human)
• Normal lung (human)
• SMRT
• Cyclin D1

Gender differences in lung disease and cancer are well-established. We reported estrogenic transcriptional responses in lung adenocarcinoma cells from females but not males despite similar estrogen receptor (ER) expression. Here we tested the hypothesis that normal human bronchial epithelial cells (HBECs) show gender-independent estrogenic responses. We report that a small sample of HBECs express similar to twice as much ER beta as ER alpha. ER alpha and ER beta were located in the cytoplasm, nucleus, and mitochondria. In contrast to lung adenocarcinoma cells, estradiol (E(2)) induced estrogen response element (ERE)-mediated luciferase reporter activity in transiently transfected HBECs regardless of donor gender. Overexpression of ER alpha-VP16 increased ERE-mediated transcriptional activity in all HBECs. E2 increased and 4-hydroxytamoxifen and ICI 182,780 inhibited HBEC proliferation and cyclin D1 expression in a cell line-specific manner. In conclusion, the response of HBECs to ER ligands is gender-independent suggesting that estrogenic sensitivity may be acquired during lung carcinogenesis.

• 出版日期2009-6-16