Acute toxicity and anaesthetic effect of clove oil and eugenol on Siamese fighting fish, Betta splendens

作者：Pattanasiri Thanapat; Taparhudee Wara; Suppakul Panuwat^*

来源：Aquaculture International, 2017, 25(1): 163-175.

DOI：10.1007/s10499-016-0020-2

• Acute toxicity
• Anaesthesia
• Clove oil
• Eugenol
• Ornamental fish
• Siamese fighting fish

With brilliant colouration and long, flowing fins, the Siamese fighting fish (Betta splendens) is one of the most popular species of freshwater aquarium fish. In the ornamental Siamese fighting fish business, stress is one of the major causes of fish injury, including collapse of the fins. This problem could be solved by using anaesthetic treatment. Clove oil and eugenol were investigated for acute toxicity, which was determined using an aqueous dilution method at concentrations ranging from 0 to 35 mg L-1. Based on a criterion of fish transportation within 2 days, induction times of anaesthesia, fish behavioural responses and mortality, and recovery times were monitored and recorded over a 48-h period. The 48-h LC50 of clove oil and eugenol was 30.63 [with a 95 % confidence interval (CI) of 29.23-32.10 mg L-1] and 29.95 mg L-1 (with a 95 % CI of 28.50-31.48 mg L-1), respectively. Concentrations of 10 and 15 mg L-1 clove oil over a 48-h period induced a sedative effect, resulting in partial loss of reactivity and mobility while maintaining equilibrium. At these concentrations, the fish could recover behaviourally within 3-5 min. Recovery times decreased with lower doses and shorter exposure times. The higher the concentration of either clove oil or eugenol used, the lower the concentrations of total ammonia and un-ionized ammonia that were detected. This study revealed that clove oil and eugenol are highly effective anaesthetic agents as a transportation mixture for Siamese fighting fish.

• 出版日期2017-2