An on-line HPLC-FRSD system for rapid evaluation of the total antioxidant capacity of Citrus fruits

作者：Zhang, Hua; Xi, Wanpeng; Yang, Yifei; Zhou, Xingyu; Liu, Xin; Yin, Shanshan; Zhang, Jinmei; Zhou, Zhiqin^*

来源：Food Chemistry, 2015, 172: 622-629.

DOI：10.1016/j.foodchem.2014.09.121

• Citrus fruits
• Total antioxidant capacity
• Evaluation
• On-line HPLC-FRSD

An on-line high performance liquid chromatography-free radical scavenging detection (HPLC-FRSD) system was developed for rapidly evaluating the total antioxidant capacity (TAC) of Citrus fruits. With the system, all samples can be analyzed within 5 min. The on-line HPLC-FRSD system has low limits of detection (0.001-0.010 mg mL(-1)) and quantification (0.005-0.020 mg mL(-1)), excellent recovery rate (90.44-115.72%), stability (RSD < 15.80%), reproducibility (RSD < 2%), and precision (RSD < 2%). Using a guard column instead of an analytic column, this new on-line HPLC-FRSD system performed better than the existing on-line HPLC methods in the analysis of the TAC of Citrus. Compared with the conventional off-line 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzthiozoline-6)-sulphonic acid (ABTS) radical scavenging methods, our newly developed system is faster and more robust. The methodology can be a good alternative for analysis TACs of Citrus fruits and potentially for other plants and plant-based products.

• 出版日期2015-4-1
• 单位西南大学