Achyranthes bidentata polysaccharides induce chondrocyte proliferation via the promotion of the G(1)/S cell cycle transition

作者：Yu Fangrong; Li Xihai; Cai Liangliang; Li Huiting; Chen Jiashou; Wong Xiaping; Xu Huifeng; Zheng Chunsong; Liu Xianxiang^*; Ye Hongzhi

来源：Molecular Medicine Reports, 2013, 7(3): 935-940.

DOI：10.3892/mmr.2013.1286

• Achyranthes bidentata polysaccharides
• chondrocyte
• cell proliferation
• osteoarthritis

Achyranthes bidentata polysaccharides (ABPS) are the major bioactive constituents of Radix Achyranthes bidentata (AB), which has been widely used in traditional Chinese medicine for the treatment of osteoarthritis. However, the molecular mechanisms behind the therapeutic effect of ABPS remain unclear. In the present study, chondrocytes were isolated from Sprague-Dawley rats. The effects of ABPS on the G(1)/S cell cycle transition in primary chondrocytes were investigated. The chondrocytes treated with and without ABPS were analyzed and it was observed that ABPS treatment was able to enhance chondrocyte proliferation in a dose- and time-dependent manner and promote the progression of chondrocyte cell cycle proliferation via the promotion of the G, to S phase transition. Furthermore, using RT-PCR and western blot analysis, ABPS were observed as significantly upregulating the expression of cyclin D1 and the cyclin-dependent kinases (CDKs) CDK4 and CDK6. These results suggest that ABPS are able to promote chondrocyte proliferation via the promotion of the G(1)/S cell cycle transition.

• 出版日期2013-3
• 单位福建中医药大学