Analysis of grain elements and identification of best genotypes for Fe and P in Afghan wheat landraces

作者：Kondou Youichi^*; Manickavele Alagu; Komatsu Kenji; Arifi Mujiburahman; Kawashima Mika; Ishii Takayoshi; Hattori Tomohiro; Iwate Hiroyoshi; Tsujimoto Hisashi; Ban Tomohiro; Matsui Minami

来源：Breeding Science, 2016, 66(5): 676-682.

DOI：10.1270/jsbbs.16041

• wheat landraces
• elements
• high-throughput method
• best genotypes

This study was carried out with the aim of developing the methodology to determine elemental composition in wheat and identify the best germplasm for further research. Orphan and genetically diverse Afghan wheat land races were chosen and EDXRF was used to measure the content of some of the elements to establish elemental composition in grains of 266 landraces using 10 reference lines. Four elements, K, Mg, P, and Fe, were measured by standardizing sample preparation. The results of hierarchical cluster analysis using elemental composition data sets indicated that the Fe content has an opposite pattern to the other elements, especially that of K. By systematic analysis the best wheat germplasms for P content and Fe content were identified. In order to compare the sensitivity of EDXRF, The sampling method for measurement using EDXRF was optimized resulting in high-throughput profiling of elemental composition in wheat grains at low cost. Using this method, we have characterized the Afghan wheat landraces improvement.

• 出版日期2016-12
• 单位RIKEN