Methanobacterium aggregans sp nov., a hydrogenotrophic methanogenic archaeon isolated from an anaerobic digester

作者：Kern Tobias; Linge Mary; Rother Michael^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2015, 65(6): 1975-1980.

DOI：10.1099/ijs.0.000210

A novel, strictly anaerobic, hydrogenotrophic methanogen, strain E09F.3(T), was isolated from a commercial biogas plant in Germany. Cells of E09F.3(T) were Gram-stain-negative, non-motile, slightly curved rods, long chains of which formed large aggregates consisting of intertwined bundles of chains. Cells utilized H-2 + CO2 and, to a lesser extent, formate as substrates for growth and methanogenesis. The optimal growth temperature was around 40 degrees C; maximum growth rate was obtained at pH around 7.0 with approximately 6.8 mM NaCl;. The DNA G+C content of strain E09F.3(T) was 39.1 mol%. Phylogenetic analyses based on 16S rRNA and mcrA gene sequences placed strain E09F.3(T). within the genus Methanobacterium. On the basis of 16S rRNA gene sequence similarity, strain E09F.3(T). was closely related to Methanobacterium congolense C-T but morphological, physiological and genomic characteristics indicated that strain E09F.3(T) represents a novel species. The name Methanobacterium aggregans sp. nov. is proposed for this novel species, with strain E09F.3(T) (=DSM 29428(T)=JCM 30569(T)) as the type strain.

• 出版日期2015-6