Dietary exposure to benzoxazinoids enhances bacteria-induced monokine responses by peripheral blood mononuclear cells

作者：Damgaard Dres; Jensen Bettina Margrethe; Palarasah Yaseelan; Nielsen Michael Friberg Bruun; Adhikari Khem Bahadur; Schnoor Heidi Julius; Juel Berg Nanna; Poulsen Lars K; Fomsgaard Inge S; Nielsen Claus Henrik^*

来源：Molecular Nutrition & Food Research, 2015, 59(11): 2190-2198.

DOI：10.1002/mnfr.201500151

• Benzoxazinoids
• Inflammation
• Lipopolysaccharide
• Monokines
• Porphyromonas gingivalis

Scope: To examine potentially immunomodulating effects of dietary benzoxazinoids (BXs), present in cereal grains.
Methods and results: Nineteen healthy volunteers were randomly distributed into two groups, who received diets with high or low content of BXs for 3 wk. After a week's wash-out, the groups switched diets. Peripheral blood mononuclear cells (PBMCs) were stimulated with Porphyromonas gingivalis, Escherichia coli lipopolysaccharide (LPS), or tetanus toxoid (TT). PBMCs from a healthy donor received the same stimuli in presence of serum from each participant receiving BXs. The production of monokines, T-cell cytokines and T-helper cell proliferation were assessed. A 3-wk diet with high BX content enhanced IL-1 beta responses against LPS and P. gingivalis, as well as TNF-alpha response against P. gingivalis, after 24 h of stimulation. Moreover, IL-6 was found to be increased after 7 days of stimulation with LPS. No effect was observed on T-cell cytokines or proliferation. BX levels in serum after a single meal did not modify cytokine responses.
Conclusion: High dietary intake of BXs enhances bacteria-induced production of pro-inflammatory monokines by PBMCs, but not T-cell responses; presumably due to intrinsic changes within PBMCs, built up over 3 wk of BX-rich diet, rather than to an immediate effects of BXs contained in serum.

• 出版日期2015-11