Membrane-binding and enzymatic properties of RPE65

作者：Kiser Philip D; Palczewski Krzysztof^*

来源：Progress in Retinal and Eye Research, 2010, 29(5): 428-442.

DOI：10.1016/j.preteyeres.2010.03.002

• Retinoid isomerase
• RPE65
• Membrane protein
• Metalloenzyme
• Retinal pigment epithelium
• Leber congenital amaurosis

Regeneration of visual pigments is essential for sustained visual function. Although the requirement for non-photochemical regeneration of the visual chromophore, 11-cis-retinal, was recognized early on, it was only recently that the trans to cis retinoid isomerase activity required for this process was assigned to a specific protein, a microsomal membrane enzyme called RPE65. In this review, we outline progress that has been made in the functional characterization of RPE65. We then discuss general concepts related to protein-membrane interactions and the mechanism of the retinoid isomerization reaction and describe some of the important biochemical and structural features of RPE65 with respect to its membrane-binding and enzymatic properties.

• 出版日期2010-9