A targetable nanogenerator of nitric oxide for light-triggered cytotoxicity

作者：Yang, Liu; Wu, Shuqi; Lin, Bijuan; Huang, Tianxun; Chen, Xiaoping; Yan, Xiaomei; Han, Shoufa^*

来源：Journal of Materials Chemistry B, 2013, 1(44): 6115-6122.

DOI：10.1039/c3tb21131b

• MANNOSE-SPECIFIC ADHERENCE
• URINARY-TRACT-INFECTIONS
• NOBEL LECTURE
• SILICA NANOPARTICLES
• ESCHERICHIA-COLI
• RELEASE
• DENDRIMERS
• PHOTODELIVERY
• SCAFFOLDS
• DISCOVERY

Nanoscale-vesicles that can target pathogens are valuable for biomedical applications. In this study, a photo-responsive nanogenerator of nitric oxide (NO) comprised of a hydrophobic core of 3trifluoromethyl- 4-nitroaniline (TFNA) and a hydrophilic shell of mannosylated poly[styrene-alter-(maleic acid)] was constructed to target and kill lectin-expressing cells. The release of NO from the nanogenerator (T@ P-M) was effectively induced by luminol-derived chemiluminescence (CL), leading to high-efficiency killing of Escherichia coli (E. coli) treated with T@ P-M. In addition, the uptake of T@ P-M by Raw 264.7 macrophages was achieved by cell surface lectin-mediated endocytosis, enabling the intracellular release of NO from the internalized T@ P-M upon the induction of extracellular chemiluminescence. Because in vivo-generated CL can overcome the limited penetration of exogenous light into biological tissues, T@ P-M has potential uses as a targetable photo-activatable microbicide to combat pathogens bearing lectins or residing in macrophages.

• 出版日期2013
• 单位厦门大学