Anti-invasive activity of histone deacetylase inhibitors via the induction of Egr-1 and the modulation of tight junction-related proteins in human hepatocarcinoma cells

作者：Kim Sung Ok; Choi Byung Tae; Choi Il Whan; Cheong Jaehun; Kim Gi Young; Kwon Taeg Kyu; Kim Nam Deuk; Choi Yung Hyun^*

来源：BMB Reports, 2009, 42(10): 655-660.

DOI：10.5483/bmbrep.2009.42.10.655

• Claudin-3
• Egr-1
• HDAC inhibitors
• Invasion
• Tight junction

The potential anti-metastasis and anti-invasion activities of early growth response gene-1 (Egr-1) and claudin-3, a tight junction (TJ)-related protein, were evaluated using histone deacetylase (HDAC) inhibitors in human hepatocarcinoma cells. The results of wound healing and Transwell assays showed that HDAC inhibitors such as trichostatin A and sodium butyrate inhibited cell migration and invasion. HDAC inhibitors markedly induced Egr-1 expression during the early period, after which expression levels decreased. in addition, the down-regulation of snail and type 1 insulin-like growth factor receptor (IGF-1R) in HDAC inhibitor,treated cells induced the upregulation of thrombospondin-1 (TSP-1), E-cadherin and claudin-3. Cells transfected with Egr-1 and claudin-3 siRNA displayed significant blockage of HDAC inhibitor-induced anti-invasive activity. Collectively, these findings indicate that the up-regulation of Egr-1 and claudin-3 are crucial steps in HDAC inhibitor-induced anti-metastasis and anti-invasion. [BMB reports 2009; 42(10): 655-660]

• 出版日期2009-10-31