Novel Streptavidin-Functionalized Silicon Nanowire Arrays for CD4(+) T Lymphocyte Separation

作者：Kim Sung Tae; Kim Dong Joo; Kim Tae Jin; Seo Deok Won; Kim Tae Hong; Lee Seung Yong; Kim Kwanghee; Lee Kyung Mi^*; Lee Sang Kwon

来源：Nano Letters, 2010, 10(8): 2877-2883.

DOI：10.1021/nl100942p

• Silicon nanowire
• streptavidin
• CD4(+) T lymphocyte
• cell separation

Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4(+) T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalizeci SiNW with biotin-labeled CD4(+) T lymphocytes. Fabricated SiNW arrays easily separated the CD4(+) T lymphocytes from the mouse whole splenocytes with over similar to 88% purity and demonstrated tight attachment to CD4(+) T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.

• 出版日期2010-8