Development and validation of a solid-phase extraction method coupled with HPLC-UV detection for the determination of biogenic amines in Chinese rice wine

作者：Zhang, Ying; Li, Yan; Zhu, Xiao-Juan; Li, Min; Chen, Hao-Yu; Lv, Xiao-Ling; Zhang, Jian^*

来源：Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure& Risk Assessment, 2017, 34(7): 1172-1183.

DOI：10.1080/19440049.2017.1317366

• Biogenic amines
• Chinese rice wine
• solid-phase extraction
• matrix effects
• high-performance liquid chromatography

A reliable and accurate method for the determination of seven biogenic amines (BAs) was developed and validated with Chinese rice wine samples. The BAs were derivatised with dansyl chloride, cleaned up using solid-phase extraction (SPE) and separated by high-performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection. The optimised derivatisation reaction, conducted at pH 9.6 and 60 degrees C for 30min, ensured baseline separation and peak symmetry for each BA. SPE clean-up using Oasis MCX cartridges yielded good recovery rates for all BAs and effectively reduced matrix effects. The developed method shows good linearity with determination coefficients of more than 0.9989 over a concentration range of 0.1-100mgl(-1). The limits of detection (LODs) for the investigated BAs ranged from 2.07 to 5.56 mu gl(-1). The intra- and inter-day relative standard deviations (RSDs) ranged from 0.86% to 3.81% and from 2.13% to 3.82%, respectively. Spiking experiments showed that the overall recovery rates ranged from 85% to 113%. Thus, the proposed method was demonstrated as being suitable for simultaneous detection, with accurate and precise quantification, of BAs in Chinese rice wine.

• 出版日期2017
• 单位天津科技大学