A highly selective fluorescent probe for direct detection and isolation of mouse embryonic stem cells

作者：Chandran Yogeswari; Kang Nam Young; Park Sung Jin; Alamudi Samira Husen; Kim Jun Young; Sahu Srikanta; Su Dongdong; Lee Jungyeol; Vendrell Marc^*; Chang Young Tae

来源：Bioorganic & Medicinal Chemistry Letters, 2015, 25(21): 4862-4865.

DOI：10.1016/j.bmcl.2015.06.037

• Mouse embryonic stem cells
• Fluorescence imaging
• Single cell PCR
• Fluorescence-assisted cell sorting
• Three germ layers

Stem cell research has gathered immense attention in the past decade due to the remarkable ability of stem cells for self-renewal and tissue-specific differentiation. Despite having numerous advancements in stem cell isolation and manipulation techniques, there is a need for highly reliable probes for the specific detection of live stem cells. Herein we developed a new fluorescence probe (CDy9) with high selectivity for mouse embryonic stem cells. CDy9 allows the detection and isolation of intact stem cells with marginal impact on their function and capabilities.

• 出版日期2015-11-1