Multicomponent adsorption of monoclonal antibodies on macroporous and polymer grafted cation exchangers

作者：Perez Almodovari Ernie X; Wu Yige; Carta Giorgio^*

来源：Journal of Chromatography A, 2012, 1264: 48-56.

DOI：10.1016/j.chroma.2012.09.064

• Monoclonal antibodies
• Multicomponent adsorption
• Pore diffusion
• Single file diffusion

We compare the rates of adsorption of two monoclonal antibodies (mAbs) with different adsorption properties on the cation exchangers UNOsphere (TM) S and Nuvia (TM) S. The former contains large open pores while the latter is based on a backbone matrix similar to UNOsphere (TM) S but also contains grafted charged polymers. Both single component and two-component adsorption are considered. Adsorption capacity and rates are much higher for Nuvia (TM) S indicating that protein interactions with the charged grafted polymers facilitate both binding and diffusional transport. Intraparticle concentration profiles obtained by confocal microscopy show sharp fronts for UNOsphere (TM) S but diffuse profiles for Nuvia (TM) S. Transport is thus controlled by pore diffusion for UNOsphere (TM) S but is described by a single file diffusion (SFD) mechanism for Nuvia (TM) S. As a result, single and two-component adsorption occur at similar rates for UNOsphere (TM) S independent of the direction for transport. For Nuvia (TM) S, however, transport is very fast for single or two-component co-adsorption but very slow when counter diffusion of the two mAbs takes place within the particles. The transport models developed in this work allow a prediction of separation performance for overloaded conditions typical of process scale applications.

• 出版日期2012-11-16