Gene Expression Analysis of Host Innate Immune Responses in the Central Nervous System following Lethal CVS-11 Infection in Mice

作者：Sugiura Naoko; Uda Akihiko; Inoue Satoshi^*; Kojima Daisuke; Hamamoto Noriko; Kaku Yoshihiro; Okutani Akiko; Noguchi Akira; Park Chun Ho; Yamada Akio

来源：Japanese Journal of Infectious Diseases, 2011, 64(6): 463-472.

The central nervous system (CNS) tissue of mice infected with the CVS-11 strain of rabies virus (RABV) was subjected to gene expression analysis using microarray and canonical pathway analyses. Genes associated with innate immunity as well as inflammatory responses were significantly up-regulated, corroborating with the previous findings obtained using attenuated viruses that did not induce a fatal outcome in infected mice. Histopathological examination showed that neurons in the cerebellum had undergone apoptosis. Although the extent of Fas ligand up-regulation was not so prominent, perforin and granzyme genes were highly expressed in the CNS of mice infected with CVS-11. The presence of perforin and granzymes both in the Purkinje cells and CD3 T lymphocytes strongly suggested that apoptosis of the former cells was induced by the latter cells.

• 出版日期2011-11