Depletion of autophagy receptor p62/SQSTM1 enhances the efficiency of gene delivery in mammalian cells

作者：Tsuchiya Megumi; Ogawa Hidesato; Koujin Takako; Kobayashi Shouhei; Mori Chie; Hiraoka Yasushi; Haraguchi Tokuko

来源：FEBS Letters, 2016, 590(16): 2671-2680.

DOI：10.1002/1873-3468.12262

• autophagy
• DNA beads
• LC3

Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here, we report a simple approach involving depletion of p62/SQSTM1 to enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was remarkably higher in p62-knockout murine embryonic fibroblast (MEF) cells compared with normal MEF cells. This higher efficiency was partially attenuated by ectopic expression of p62. Furthermore, siRNA-mediated knockdown of p62 clearly increased the efficiency of transfection of murine embryonic stem (mES) cells and human HeLa cells. These data indicate that p62 acts as a key regulator of gene delivery.

• 出版日期2016-8