A method to identify new molecular markers for assessing minimal residual disease in acute leukemia patients

作者：Jancuskova Tereza; Plachy Radek; Stika Jiri; Zemankova Lucie; Hardekopf David W; Liehr Thomas; Kosyakova Nadezda; Cmejla Radek; Zejskova Lenka; Kozak Tomas; Zak Pavel; Zavrelova Alzbeta; Havlikova Pavlina; Karas Michal; Junge Annelore; Ramel Christian; Pekova Sona^*

来源：Leukemia Research, 2013, 37(10): 1363-1373.

DOI：10.1016/j.leukres.2013.06.009

• Acute leukemia
• Minimal residual disease
• Cytogenetics
• Chromosome microdissection
• Next-generation sequencing
• Personalized medicine

Acute leukemias (AL) comprise a heterogeneous group of hematologic malignancies, and individual patient responses to treatment can be difficult to predict. Monitoring of minimal residual disease (MRD) is thus very important and holds great potential for improving treatment strategies. Common MRD targets include recurrent cytogenetic abnormalities and mutations in important hematological genes; unfortunately well-characterized targets are lacking in many AL patients. Here we demonstrate a technical approach for the identification and mapping of novel clone-specific chromosomal abnormalities down to the nucleotide level. We used molecular cytogenetics, chromosome microdissection, amplification of the microdissected material, and next-generation sequencing to develop PCR-based MRD assays based on unique breakpoint sequences.

• 出版日期2013-10