A simple highly selective and sensitive hydroquinone-based two-photon fluorescent probe for imaging peroxynitrite in live cells

作者：Zhu, Baocun^*; Wang, Zuokai; Zhao, Ziyang; Shu, Wei; Zhang, Meng; Wu, Liu; Liu, Caiyun^*; Duan, Qingxia; Jia, Pan

来源：Sensors and Actuators B: Chemical , 2018, 262: 380-385.

DOI：10.1016/j.snb.2018.01.203

• Fluorescent probe
• Peroxynitrite (ONOO-)
• Hydroquinone
• Hydroxynaphthalimide
• Two-photon bioimaging

Exploiting specific and sensitive techniques for tracking peroxynitrite (ONOO-) in the biological systems is of great significance to understand its diverse pathophysiology. Herein, we have constructed a simple, 4-hydroxynaphthalimide-derived two-photon fluorescent probe TPHQ for imaging ONOO- in live cells. Probe TPHQ with the recognition receptor of hydroquinone moiety exhibited excellent selectivity towards ONOO- over various bio-related analytes including H2O2 and OCl-. Additionally, probe TPHQ could rapidly monitor ONOO- with high sensitivity (DL = 16 nM). With probe TPHQ the fluctuations of ONOO- levels in live cells were successfully tracked by the one-photon and two-photon fluorescence microscopy.

• 出版日期2018-6-1
• 单位济南大学