Water and H2O2 Permeability of Aquaporins Isolated from Cucumber and Fig leaf Gourd

作者：Rhee Ji Ye; Katsuhara Maki; Kang Hunseung; Chung Gap Chae^*

来源：Horticulture Environment and Biotechnology, 2010, 51(3): 167-172.

• hydraulic conductivity
• osmotic water permeability
• Xenopus oocyte

Cucumber aquaporin gene, CsPIP1;1 and figleaf gourd aquaporin gene CfPIP2;1 were injected to Xenopus oocytes in order to study water transport activity. While CsPIP1;1 did not transport any water and was not responsive to mercury, oocyte injected with CfPIP2;1 rapidly increased their volume without saturation indicating active water transport. Calculation of water permeability of oocytes clearly distinguished the two genes. In response to exogenous application of H2O2, only CfPIP2;1 responded, reducing osmotic water permeability. Transgenic Arabidopsis plants overexpressing aquaporin genes did not respond to applied H2O2.

• 出版日期2010-6