Multivalent Aptamer/Gold Nanoparticle-Modified Graphene Oxide for Mass Spectrometry-Based Tumor Tissue Imaging

作者：Huang Rong Cing; Chiu Wei Jane; Lai Irving Po Jung; Huang Chih Ching^*

来源：Scientific Reports, 2015, 5(1): 10292.

DOI：10.1038/srep10292

The protein mucin1 (MUC1) is an attractive target for cancer biomarkers because it is overexpressed in most adenocarcinomas. In this study, we exploited a MUC1-binding aptamer (Apt(MUC1)) as a targeting agent for nanoparticle-based imaging systems coupled with laser desorption/ionization mass spectrometry (LDI-MS). We found that Apt(MUC1)-conjugated gold nanoparticles immobilized, through hydrophobic and pi-pi interactions, on graphene oxide (Apt(MUC1)-Au NPs/GO) bound effectively to MUC1 units on tumor cell membranes. The ultrahigh density and high flexibility of AptMUC1 on the GO surface enhanced the platform's cooperative and multivalent binding affinity for MUC1 on cell membranes. After we had labeled MUC1-overexpressing MCF-7 cells (human breast adenocarcinoma cell line) with Apt(MUC1)-Au NPs/GO, we used LDI-MS to monitor Au cluster ions ([Au-n](+); n = 1-3), resulting in the detection of as few as 100 MCF-7 cells. We also employed this Apt(MUC1)-Au NPs/GO-LDI-MS system to analyze four different MUC1 expression cell lines. In addition, the Apt(MUC1)-Au NPs/GO platform could be used further as a labeling agent for tumor tissue imaging when coupled with LDI-MS. Thus, Apt-Au NPs/GO can function as a highly amplified signal transducer through the formation of large Au clusters ions during LDI-MS analysis.

• 出版日期2015-5-14