A Rapid and Economical Method for Low Molecular Weight RNA Isolation from a Wide Variety of Plant Species

作者：An, Zewei; Li, Yachao; Xie, Lili; Zhai, Qilin; Huang, Huasun^*

来源：Bioscience Biotechnology and Biochemistry, 2013, 77(7): 1599-1601.

DOI：10.1271/bbb.130187

• low molecular weight RNA
• microRNA
• polyethylene glycol
• plant

We have developed a time- and cost-effective method for isolating low molecular weight (LMW) RNA from plants. In our protocol, the isolation procedure can be completed within 3 h. Polyethylene glycol (PEG) and absolute ethanol are used to isolate LMW RNA, and the LMW RNA yields were >80 mu g/g of fresh-weight tissues for several of the plant species tested.

• 出版日期2013-7
• 单位中国热带农业科学院; 海南大学