Affibody molecule-mediated depletion of HSA and IgG using different buffer compositions: a 15 min protocol for parallel processing of 1-48 samples

作者：Eriksson Cecilia; Schwenk Jochen M; Sjoberg Anna; Hober Sophia^*

来源：Biotechnology and Applied Biochemistry, 2010, 56(2): 49-57.

DOI：10.1042/BA20100041

• Affibody molecule
• human serum albumin (HSA)
• IgG
• protein depletion
• high throughput analysis
• sample preparation
• serum or plasma analysis

High-abundant plasma proteins pose a challenge in a large number of proteomics-based technologies. Depletion of these high-abundant proteins has proven to be a fruitful strategy to circumvent masking of lower-abundant proteins that could serve as valuable biomarker candidates. However, current strategies often do not meet the throughput requirements of large-scale proteomic studies. In the present paper, a flexible and parallelized method for the depletion of high-abundant proteins is described, allowing the removal of the two most abundant proteins from 48 blood-derived samples in less than 15 min using Affibody molecules as affinity ligands. A sample-processing platform like this should be suitable for a number of proteomics technologies; its flexibility in buffer composition allows for different types of downstream applications.

• 出版日期2010-6