Limb Remote Ischemic Conditioning Promotes Myelination by Upregulating PTEN/Akt/mTOR Signaling Activities after Chronic Cerebral Hypoperfusion

作者：Li, Xiaohua; Ren, Changhong; Li, Sijie; Han, Rongrong; Gao, Jinhuan; Huang, Qingjian; Jin, Kunlin; Luo, Yinghao^*; Ji, Xunming^*

来源：Aging and Disease, 2017, 8(4): 392-401.

DOI：10.14336/AD.2016.1227

• cerebral hypoperfusion
• white matter
• vascular dementia
• oligodendrocyte
• ischemic conditioning
• PI3K/Akt/mTOR

Limb Remote ischemic conditioning (LRIC) has been proved to be a promising neuroprotective method in white matter lesions after ischemia; however, its mechanism underlying protection after chronic cerebral hypoperfusion remains largely unknown. Here, we investigated whether LRIC promoted myelin growth by activating PI3K/Akt/mTOR signal pathway in a rat chronic hypoperfusion model. Thirty adult male Sprague Dawley underwent permanent double carotid artery (2VO), and limb remote ischemic conditioning was applied for 3 days after the 2VO surgery. Cognitive function, oligodendrocyte counts, myelin density, apoptosis and proliferation activity, as well as PTEN/Akt/mTOR signaling activity were determined 4 weeks after treatment. We found that LRIC significantly inhibited oligodendrocytes apoptosis (p< 0.05), promoted myelination (p< 0.01) in the corpus callosum and improved spatial learning impairment (p< 0.05) at 4 weeks after chronic cerebral hypoperfusion. Oligodendrocytes proliferation, along with demyelination, in corpus callosum were not obviously affected by LRIC (p> 0.05). Western blot analysis indicated that LRIC upregulated PTEN/Akt/mTOR signaling activities in corpus callosum (p< 0.05). Our results suggest that LRIC exerts neuroprotective effect on white matter injuries through activating PTEN/Akt/mTOR signaling pathway after chronic cerebral hypoperfusion.

• 出版日期2017-7
• 单位首都医科大学