科研之友
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摘要
Bilirubin oxidase (BOX) from Myrothecium sp. IMER1 was immobilized on chitosan using glutaraldehyde as a cross-linking agent. FTIR spectra of the chitosan before and after BOX immobilization proved that the enzyme had been successfully immobilized to the cross-linked chitosan. The stabilities of the immobilized BOX were improved compared to free counterpart. The apparent K-m for the immobilized enzyme was 1,097.1 mu M, which increased by about 1.74-fold compared to the free enzyme. The immobilized BOX was operated in a batch mode for the decolorization of indigo carmine from aqueous solution. Decolorization rates of the dye by the immobilized BOX (92%) were higher than that by the free enzyme (83%) in 150 mm. Immobilized BOX could be used repeatedly, and its removal efficiency for indigo carmine remained about 79% for up to ten usages. The results demonstrated that immobilized BOX has potential application in dyestuff treatment.
