Distinguishing Cancerous Liver Cells Using Surface-Enhanced Raman Spectroscopy

作者：Huang, Jing; Liu, Shupeng^*; Chen, Zhenyi; Chen, Na; Pang, Fufei; Wang, Tingyun

来源：Technology in Cancer Research and Treatment, 2016, 15(1): 36-43.

DOI：10.1177/1533034614561358

• gold nanoparticles
• SERS
• cancerous liver cells

Raman spectroscopy has been widely used in biomedical research and clinical diagnostics. It possesses great potential for the analysis of biochemical processes in cell studies. In this article, the surface-enhanced Raman spectroscopy (SERS) of normal and cancerous liver cells incubated with SERS active substrates (gold nanoparticle) was measured using confocal Raman microspectroscopy technology. The chemical components of the cells were analyzed through statistical methods for the SERS spectrum. Both the relative intensity ratio and principal component analysis (PCA) were used for distinguishing the normal liver cells (QSG-7701) from the hepatoma cells (SMMC-7721). The relative intensity ratio of the Raman spectra peaks such as I-937/I-1209, I-1276/I-1308, I-1342/I-1375, and I-1402/I-1435 was set as the judge boundary, and the sensitivity and the specificity using PCA method were calculated. The results indicated that the surface-enhanced Raman spectrum could provide the chemical information for distinguishing the normal cells from the cancerous liver cells and demonstrated that SERS technology possessed the possible applied potential for the diagnosis of liver cancer.

• 出版日期2016-2
• 单位上海大学