Advancing Biocapture Substrates via Chemical Lift-Off Lithography

作者：Cao Huan H; Nakatsuka Nako; Liao Wei Ssu; Serino Andrew C; Cheunkar Sarawut; Yang Hongyan; Weiss Paul S^*; Andrews Anne M^*

来源：Chemistry of Materials, 2017, 29(16): 6829-6839.

DOI：10.1021/acs.chemmater.7b01970

Creating small-molecule-functionalized platforms for high-throughput screening or biosensing applications requires precise placement of probes on solid substrates and the ability to capture and to sort targets from multicomponent samples. Here, chemical lift-off lithography was used to fabricate large-area, high-fidelity patterns of small-molecule probes. Liftoff lithography enables biotin streptavidin patterned recognition with feature sizes ranging from micrometers to below 30 rim. Subtractive patterning via lift-off facilitated insertion of a different type of molecule and, thus, multiplexed side-by-side placement of small-molecule probes such that binding partners were directed to cognate probes from solution. Small molecules mimicking endogenous neurotransmitters were patterned using lift-off lithography to capture native membrane-associated receptors. We characterized patterning of alkanethiols that self-assemble on Au having different terminal functional groups to expand the library of molecules amenable to lift-off lithography enabling a wide range of functionalization chemistries for use with this simple and versatile patterning method.

• 出版日期2017-8-22