An inexpensive high-throughput nuclear magnetic resonance tube cleaning apparatus

作者：Zhang, Bo; Hodgson, James; Hancock, Walter; Powers, Robert^*

来源：Analytical Biochemistry, 2011, 416(2): 234-236.

DOI：10.1016/j.ab.2011.05.012

• DRUG DISCOVERY
• DESIGN
• AUTOMATION

Large-scale nuclear magnetic resonance (NMR) tube cleaning is currently a bottleneck in high-throughput NMR ligand affinity screens. Expensive alternatives include discarding the NMR tubes after a single use (similar to US $2-$8/tube), using commercial NMR tube cleaners (similar to$15,000), and abandoning NMR tubes for flow probe technology (similar to$75,000). Instead, we describe a relatively inexpensive (similar to$400) and easily constructed apparatus that can clean 180 NMR tubes per hour while using a modest amount of solvent. The application of this apparatus significantly shortens the time to recycle NMR tubes while avoiding crosscontamination and tube damage.

• 出版日期2011-9-15