摘要

Changes in sucrose metabolism in response to salt (NaCl) and water (polyethylene glycol, PEG6000) iso-osmotic stresses were measured in tomato cultivar Liaoyuan Duoli (Solanum lycopersicum L.) and the objective was to provide a new evidence for the relationship between salt and osmotic stresses. The carbohydrate contents, as well as sucrose metabolizing enzymes activities and transcript levels were determined. The results indicated that soluble sugar and hexoses accumulated to higher levels and the contents of sucrose and starch were lower in mature fruit under the two stress treatments. Salt and water stresses can enhance the invertase and sucrose synthase activities of tomato fruit in a long period of time (45-60 days after anthesis), and elevate the expression of soluble acid invertase mRNA. It showed that two different stresses could also regulate the soluble acid invertase activity by controlling its gene expression. The activity of sucrose synthase was linked to the changes in soluble sugar levels but not with transcript levels. The effects of salt and water stress treatments on sucrose phosphate synthase activities were weak.