Dual-label quantum dot-based immunoassay for simultaneous determination of Carbadox and Olaquindox metabolites in animal tissues

作者：Le, Tao^*; Zhu, Liqian; Yu, Huan

来源：Food Chemistry, 2016, 199: 70-74.

DOI：10.1016/j.foodchem.2015.11.116

• Simultaneous detection
• Quantum dots
• Quinoxaline-2-carboxylic acid
• Methyl-3-quinoxaline-2-carboxylic acid
• Fluoroimmunoassay

A novel and reliable dual-label direct competitive fluorescence-linked immunosorbent assay (dc-FLISA) based on quantum dots (QDs) was developed for the simultaneous determination of the major metabolites of Carbadox and Olaquindox residues in animal tissues, using anti-QCA monoclonal antibodies and anti-MQCA polyclonal antibodies labeled with QD520 and QD635, respectively. The limits of detection for QCA and MQCA were 0.05 and 0.07 ng/ml, respectively. The method was used to analyze fortified samples and analyte recoveries ranged from 81.5% to 98.2% (QCA) and 84.2% to 95.7% (MQCA). Good correlations between the dc-FLISA method and HPLC were demonstrated for the determination of QCA and MQCA residues in swine tissue samples, confirming the reliability of the proposed method.

• 出版日期2016-5-15
• 单位重庆师范大学; 扬州大学