Direct electrochemistry of hemoglobin and its electrocatalytic effect based on its direct immobilization on carbon ionic liquid electrode

作者：Safavi Afsaneh^*; Maleki Norouz; Moradlou Omran; Sorouri Mohsen

来源：Electrochemistry Communications, 2008, 10(3): 420-423.

DOI：10.1016/j.elecom.2007.12.026

• hemoglobin
• carbon ionic liquid electrode
• electrocatalysis
• oxygen
• hydrogen peroxide

Direct electrochemistry of hemoglobin (Hb) has been achieved by its direct immobilization on carbon ionic liquid electrode (CILE). CILE was immersed in a solution containing Hb and ionic liquid, octylpyridinium chloride ([OcPy][Cl]), to directly immobilize Hb on CILE. Cyclic voltammetry of modified electrode exhibited quasi-reversible peaks corresponding to Hb. The oxidation and reduction peak potentials of immobilized Hb in acetate buffer solution, pH 5.0 and at a scan rate of 0.1 V s(-1) were obtained at about -150 mV and -290 mV, respectively. The average surface coverage of the electroactive Hb adsorbed on the electrode surface was calculated as 8.4 x 10(-11) mol cm(-2). Hb retained its bioactivity on modified electrode and showed excellent electrocatalytic activity towards oxygen, hydrogen peroxide and nitrite. Hydrogen peroxide can be determined in the range of 1.0 x 10(-4)-5.0 x 10(-3) M.

• 出版日期2008-3