摘要
A series of nitric oxide (NO) sensitive polymeric hydrogels have been synthesized and their properties described. The new materials are based on a poly(2-hydroxyethyl methacrylate) matrix entrapping the DAF-FM (4-amino-5-methylamino-2%26apos;,7%26apos;-difluorofluorescein) fluorescent probe. Some of the developed materials show sensitivities in the range ca. 50-75 nM towards NO dissolved in aerated phosphate buffer (PBS), being nitrous anhydride the reactive species under such conditions. Remarkably, the sensitivity of the fluorescent polymers is not influenced by dehydroascorbic acid, a molecule of biological importance, which has been reported to be a common interfering species capable to react with well known probes for NO.
- 出版日期2014-6