Post-Synthetic Defucosylation of AGP by Aspergillus nidulans alpha-1,2-Fucosidase Expressed in Arabidopsis Apoplast Induces Compensatory Upregulation of alpha-1,2-Fucosyltransferases

作者：Pogorelko Gennady V; Reem Nathan T; Young Zachary T; Chambers Lauran; Zabotina Olga A^*

来源：PLos One, 2016, 11(7): e0159757.

DOI：10.1371/journal.pone.0159757

Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant. Besides mechanical function providing cell shape, cell walls participate in intercellular communication, defense during plant-microbe interactions, and plant growth. The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes. Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations. Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes. These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.

• 出版日期2016-7-22