Dynamic transcriptional signatures and network responses for clinical symptoms in influenza-infected human subjects using systems biology approaches

作者：Linel Patrice; Wu Shuang; Deng Nan; Wu Hulin^*

来源：Journal of Pharmacokinetics and Pharmacodynamics, 2014, 41(5): 509-521.

DOI：10.1007/s10928-014-9365-1

• Gene regulatory network
• Differential equation
• ODE modeling
• Functional principle component analysis
• Dynamic response gene
• Variable selection

Recent studies demonstrate that human blood transcriptional signatures may be used to support diagnosis and clinical decisions for acute respiratory viral infections such as influenza. In this article, we propose to use a newly developed systems biology approach for time course gene expression data to identify significant dynamically response genes and dynamic gene network responses to viral infection. We illustrate the methodological pipeline by reanalyzing the time course gene expression data from a study with healthy human subjects challenged by live influenza virus. We observed clear differences in the number of significant dynamic response genes (DRGs) between the symptomatic and asymptomatic subjects and also identified DRG signatures for symptomatic subjects with influenza infection. The 505 common DRGs shared by the symptomatic subjects have high consistency with the signature genes for predicting viral infection identified in previous works. The temporal response patterns and network response features were carefully analyzed and investigated.

• 出版日期2014-10