Dynamic and Reversible Fluorescence Imaging of Superoxide Anion Fluctuations in Live Cells and in Vivo

作者：Zhang, Wen; Li, Ping; Yang, Fan; Hu, Xiufen; Sun, Chuanzhi; Zhang, Wei; Chen, Dezhan; Tang, Bo^*

来源：Journal of the American Chemical Society, 2013, 135(40): 14956-14959.

DOI：10.1021/ja408524j

• ISCHEMIA-REPERFUSION
• PROBE
• RADICALS
• STRESS
• MICROSCOPY
• REACTIVITY

Overgeneration of reactive oxygen species (ROS) is closely associated with cellular damage and diseases. As superoxide anion (O-2(center dot-)) is the precursor of other ROS, exploring O-2(center dot-). fluctuations in cells and in vivo is of great significance. To address this critical need, we have developed a novel reversible fluorescent probe with one-photon and two-photon fluorescence properties, which is well suited for monitoring O-2(center dot-) fluxes selectively and dynamically. Imaging results substantiate dynamic and reversible fluorescence responses of this probe to intracellular O-2(center dot-) under apoptotic stimuli. Moreover, this probe can conveniently visualize changes in O-2(center dot-). concentration during reperfusion injury in hepatocytes, zebrafish, and mice, by means of one-photon or two-photon imaging according to depths of various samples. The present study provides a powerful fluorescent imaging tool for dynamic tracking of O-2(center dot-). in live cells and in vivo.

• 出版日期2013-10-9
• 单位山东师范大学