AKT signalling and mitochondrial pathways are involved in mushroom polysaccharide-induced apoptosis and G(1) or S phase arrest in human hepatoma cells

作者：OuYang, Fengju; Wang, Guibin; Guo, Wei; Zhang, Yuanyuan; Xiang, Wenhua; Zhao, Min^*

来源：Food Chemistry, 2013, 138(4): 2130-2139.

DOI：10.1016/j.foodchem.2012.10.047

• Medicinal mushrooms
• Apoptosis
• Cell cycle
• Human hepatoma cells

This study describes molecular mechanisms for inhibiting tumour cell proliferation using polysaccharides from medicinal mushrooms in human hepatoma cells. The results show that regarding cell cycle-related proteins, three types of polysaccharides significantly enhance the expression of p27(Kip) in HepG2 and Bel-7404 cells, while suppressing the activity of cyclin D1/CDK4 and/or cyclin E/CDK2. Considering apoptosis-related factors, the polysaccharides suppressed ART activity through the inhibition of ART phosphorylation at Thr(308) and/or Ser(473). The growth of HepG2 and Bel-7404 cells was suppressed by the up-regulation of a subunit of PI3K and phospho-PTEN, which are modulators of AKT activity. The polysaccharides also activated the mitochondria-mediated apoptosis pathway by stimulating the activation of BcI-2 family proteins to release cytochrome c and Smac and cleave caspase-9 and caspase-3 in HepG2 and Bel-7404 cells. These factors have a potent effect on cell cycle arrest in G(1) and/or S phase and induce apoptosis in HepG2 and Bel-7404 cells.

• 出版日期2013-6-15
• 单位东北林业大学; 中国农业科学院哈尔滨兽医研究所