Development of oral CTL vaccine using a CTP-integrated Sabin 1 poliovirus-based vector system

作者：Han Seung Soo; Lee Jinjoo; Jung Yideul; Kang Myeong Ho; Hong Jung Hyub; Cha Min Suk; Park Yu Jin; Lee Ezra; Yoon Cheol Hee; Bae Yong Soo^*

来源：Vaccine, 2015, 33(38): 4827-4836.

DOI：10.1016/j.vaccine.2015.07.072

• Poliovirus Sabin 1 vector
• RPS-CTP CTL vector
• HIV-1 p24
• Recombinant poliovirus
• p24-specific CTL
• Oral CTL vaccine

We developed a CTL vaccine vector by modification of the RPS-Vax system, a mucosal vaccine vector derived from a poliovirus Sabin 1 strain, and generated an oral CTL vaccine against HIV-1.A DNA fragment encoding a cytoplasmic transduction peptide (CTP) was integrated into the RPS-Vax system to generate RPS-CTP, a CTL vaccine vector. An HIV-1 p24 cDNA fragment was introduced into the RPS-CTP vector system and a recombinant poliovirus (rec-PV) named vRPS-CTP/p24 was produced. vRPS-CTP/p24 was genetically stable and efficiently induced Th1 immunity and p24-specific CTLs in immunized poliovirus receptor-transgenic (PVR-Tg) mice. In challenge experiments, PVR-Tg mice that were pre-immunized orally with vRPS-CTP/p24 were resistant to challenge with a lethal dose of p24-expressing recombinant vaccinia virus (rMVA-p24). These results suggested that the RPS-CTP vector system had potential for developing oral CTL vaccines against infectious diseases.

• 出版日期2015-9-11