Development of a new analytical tool for assessing the mutagen 2-methyl-1, 4-dinitro-pyrrole in meat products by LC-ESI-MS/MS

作者：Molognoni Luciano; Daguer Heitor; de Sa Ploencio Leandro Antunes; Yotsuyanagi Suzana Eri; da Silva Correa Lemos Ana Lucia; Joussef Antonio Carlos; Lindner Juliano De Dea^*

来源：Talanta, 2018, 185: 151-159.

DOI：10.1016/j.talanta.2018.03.035

• Analytical validation
• Commission Decision 2002/657/EC
• DNMP
• Food safety
• Food preservatives
• Measurement uncertainty

The use of sorbate and nitrite in meat processing may lead to the formation of 2-methyl-1,4-dinitro-pyrrole (DNMP), a mutagenic compound. This work was aimed at developing and validating an analytical method for the quantitation of DNMP by liquid chromatography-tandem mass spectrometry. Full validation was performed in accordance to Commission Decision 2002/657/EC and method applicability was checked in several samples of meat products. A simple procedure, with low temperature partitioning solid-liquid extraction, was developed. The nitrosation during the extraction was monitored by the N-nitroso-DL-pipecolic acid content. Chromatographic separation was achieved in 8 min with di-isopropyl-3-aminopropyl silane bound to hydroxylated silica as stationary phase. Samples of bacon and cooked sausage yielded the highest concentrations of DNMP (68 /- 3 and 50 /- 3 mu g kg(-1), respectively). The developed method proved to be a reliable, selective, and sensitive tool for DNMP measurements in meat products.

• 出版日期2018-8-1