An Novel Strategy for Brucella Differential Vaccine Combined with Colloidal Gold Immunochromatographic Strips Based on Mutants of Brucella melitensis M5-90

作者：Zhang, Huan; Guo, Liping; Wei, Qingqing; Zhang, Ke; Zhao, Shanahan; Li, Hongyu; Chen, Chuangfu; Zhang, Wanjiang; Zhang, Yali; Wang, Yuanzhi^*

来源：Kafkas Universitesi Veteriner Fakultesi Dergisi, 2017, 23(3): 403-408.

DOI：10.9775/kvfd.2016.16803

• Brucella melitensis
• Distinguish
• Vaccine
• Colony forming units
• ICS

There is a requirement to develop an novel vaccine to distinguish between vaccinated and infected animals after vaccination. Two mutants of Brucella melitensis M5-90 Delta bp261 and M5-90 Delta bp262, knockout of the fragments amino acids 55-152 and 22-185 respectively, were primarily generated. Then the two mutants were inoculated 7 weeks old BALB/c mice with 3.0 x 10(6) CFU/0.2 mL parent or mutant strains, Serum samples were evaluated by Rose Bengal plate tests, serum agglutination tests, colloidal gold immunochromatographic strips (ICS) coated with VirB5 or BP26 and indirect enzyme-linked immunosorbent assays. Spleen tissue samples were used to determine B. melitensis abundance at 10, 20, 30, and 40 days post-immunization (dpi). The serological results showed that the parent and mutant strains elicited a weak immunological reaction at 10-30 dpi. Fewer colony forming units (CFUs) were recovered from spleens in mutant strains group than the parent strain group during 20-40 post-immunization (dpi). The ICS were able to distinguish between the sera from mice immunized with either a parent or mutant strain of B. melitensis. These results indicate that the two mutants strain of B. melitensis have potentially perspectives in distinguishing infected animals from vaccination combined with ICS in this study.

• 出版日期2017-6
• 单位平顶山学院; 石河子大学