AN ELECTROCHEMICAL BIOSENSOR BASED ON THE TYROSINASE ENZYME FOR THE DETERMINATION OF PHENOL IN WASTEWATER

作者：de Oliveira Dejane P C^*; Ribeiro Francisco W P; Becker Helena; Lima Neto Pedro; Correia Adriana N

来源：Quimica Nova, 2015, 38(7): 924-931.

DOI：10.5935/0100-4042.20150086

• biosensor
• tyrosinase
• phenol
• wastewater
• square-wave voltammetry

This work describes the development of a biosensor based on the tyrosinase enzyme (Tyr) for the determination of phenol (PHEN) in laboratory effluent samples derived from ammoniacal nitrogen analysis of the water samples from the Muquem dam in the city of Carius, CE, using square-wave voltammetry (SWV). The electrode modification consisted of the immobilization of gold nanoparticles, multi-walled carbon nanotubes, cobalt phthalocyanine, and Tyr on a glassy carbon electrode. The electrolyte, pH, enzyme quantity, and voltammetric parameters were optimized to detect PHEN. The analytical curves presented a linear range from 4.97 x 10(-6) mol L-1 to 6.10 x 10(-5) mol L-1, and the detection limit (DL) and quantitation limit (QL) values were 4.81 x 10(-6) mol L-1 and 4.97 x 10(-6) mol L-1, respectively. The repetition of measurements with the same biosensor and repetition for three other prepared biosensors exhibited a relative standard deviation (RSD) of 5.50 and 1.75%, respectively. The percentage recovery of PHEN in effluent samples varied from 86.40 to 105.04%. The stability of the biosensor was evaluated (at 21 days) with satisfactory results, showing 97.86% of the initial response. Moreover, the DL and recovery percentages agreed with the established values from CONAMA and ABNT, respectively. Thus, the electrode configuration developed seems a promising tool in the detection and quantification of PHEN in complex samples.

• 出版日期2015-8