Novel Chitosan-HEMA-Gelatin Macroporous Scaffold for Bone Tissue Engineering

作者：Singh Deepti; Bae Youmin; Singh Dolly; Won Son Tae; Kim Joon Ho; Han Sung Soo

来源：Journal of Biomaterials and Tissue Engineering, 2015, 5(6): 479-485.

DOI：10.1166/jbt.2015.1342

• Bone Tissue Engineering
• Chitosan
• 2-Hydroxyethylmethacrylate
• Gelatin
• Macroporous Matrix
• Osteoblast

To achieve repair or restore any injured organ it is pivotal to mimic natural system in the in-vitro conditions and understand the critical parameter that leads to in-vivo tissue restoration. In this study, we have for the first time fabricated chitosan-2-hydroxyethyl (methacrylate)-gelatin (CHG) composite as an artificial ECM for tissue engineering applications. Scaffold was tested for its structural and architecture properties using Micro-CT, TG, DSC and degradation assays. Micro-CT testing shows pore diameter for CHG scaffold were in range of 50-150 mu m with average pore diameter of 100 mu m. Furthermore, analysis reveals highly porous interconnected CHG matrix. For checking the ability of scaffold to support cells growth, human osteoblasts were cultured for 4 weeks. Cell viability assay performed shows CHG scaffold support higher cellular proliferation rate and DAPI staining performed to check for cell matrix interplay shows consistent increase in the cell numbers for over 3 weeks. Overall, CHG scaffold was found to be mechanically stable and architecture suitable for osteoblast attachment, proliferation, was able to support cellular mineralization and could be further explored as potential scaffold for bone and musculoskeletal tissue engineering applications.

• 出版日期2015-6